PDF | The present investigation was carried out to evaluate the antioxidant nature of ethanolic extract of Terminalia arjuna bark (EETA) on. Bioactive Components and Antioxidant Properties of Terminalia arjuna L. Extracts . Shahid Chatha, Shahzad Ali, Department of Applied Chemistry. Barks extracts of four different trees (Azadirachta indica, Terminalia arjuna, Acacia Antioxidant activity (AA) was determined by measuring reducing power, .
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The different extracts from arjuna exhibited a close dependent reducing power and it was observed that reducing power of extracts from different extracts varied to some extent and found in the range of 0. The earlier investigations in the relevant field had clearly demonstrated that methanol was a promising solvent for extraction of antioxidants from natural products [ 16 ]. K at ambient temperature following the arkuna established method [ 4 ].
Percent inhibition of linoleic acid peroxidation by synthetic antioxidant BHT was taken as control and compared with arjuna extracts. Research Article Open Access.
February 18, ; Published Date: The plant Terminalia arjuna L. Moreover, there is a growing awareness among consumers regarding food additive safety [ 5 ].
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Ethanol showed relatively low yields as compared to methanol, however, ethanol is even then preferred solvent to extract antioxidant compounds due to it lower toxicity. All the chemicals and reagents used in this research were of analytical grade and used were obtained from Sigma Chemical Co.
The degree of oxidation was measured following ammonium thiocyanate method [ 15 ]. The arjuna extracts are found to be quite effective in preventing the terimnalia. Sample containing synthetic antioxidant, BHT was used as positive control. The antioxidant capacities of arjuna extracts were also evaluated by measuring their free radical scavenging abilities to 1,1 —diphenyl-2 picrylhydrazyl DPPH stable following the previously established method [ 14 ] with slight modifications.
Bioactive Components and Antioxidant Properties of Terminalia arjuna L.Extracts
Our finding regarding flavonoids in the present study were comparable with the findings antioxidatn other antioxodant [ 16 ] worked on antioxidant activity of different plants including Acacia nilotica, Terminalia arjuna, Eugenia jambolana and Azadirachta indica using different solvent blends of ethanol, methanol and water.
The sample containing butylated hydroxytoulene BHT was used as a positive control. Extraction yield was dependant on the chemical composition of the bark of tree, as the phenolic and flavonoids contents investigated in the bark extracts Terminalia arjuna from different areas [ 18 ]. In Ayurvedic system, it is styptic, anthelmintic, alexiteric, tonic, and useful in fractures, heart diseases, urinary discharges, biliousness, ulcers, asthma, tumors, anemia and excessive perspiration etc.
J Food Process Technol 5: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The percentage yield of the different arjuna extracts was found in the range of 6. The amounts of total phenolic were calculated using a calibration curve Gallic terminaoia ppm. The percentage yield of the extracts was calculated by using the following formula.
The terminaalia water in the plant material was removed using paper towel by pressing on them gently. Activity of extract increases with the increase in concentration of phenolic compounds. The oxidation reactions can produce free radicals, which start chain reactions and subsequently can cause a large number of cardiovascular diseases in humans [ 1 ]. Antioxidant compounds terminate these chain reactions by removing free radical intermediates, and inhibit other oxidation reactions by being oxidized themselves [ 2 ].
Bioactive Components and Antioxidant Properties of Terminalia arjuna L. Extracts
Authenticated samples of leaves and bark were separately packed in polythene bags and transferred to the Natural Products Research Laboratory, Government College University Faisalabad. The maximum amount of flavonoids 5. Therefore, this project was designed to investigate bioactive components phenolics and flavonoidsfree radical scavenging property and antioxidant activity of extracts from barks and leaves of arjuna plant indigenous to Pakistan.
Briefly, 15 gram of ground material was taken in conical flask followed by the addition of mL of each solvent separately. The mixture was kept at room temperature for 10 minutes, and then 1. Sample containing BHT served as ariuna control. TFC amounts were expressed as catechin equivalents per hundred grams of dry matter. The results suggested that extracts of Terminalia arjuna L. Agri anfioxidant Aquaculture Journals Dr. For extraction of flavonoids compounds, ethanol has been proved very appropriate solvents due to their low toxicity [ 13 ].
The absorbance of the resulting solutions and the blank were recorded after 1 hour at room temperature. Pharmaceutical Sciences Journals Ann Jose ankara escort. Upon arrival in the laboratory the leaves and stem bark samples were washed thoroughly with tap water to remove any wastes and dust particles. Percent inhibition of linoleic acid was calculated as. The reducing power was found to be increasing by increasing the concentration of extracts.
Two different solvent systems aqueous ethanol water: The data regarding the total flavonoid contents, 1. The reducing power of a compound is related to its electron transfering ability and may serve as a wntioxidant indicator of its antioxidant activity [ 20 ]. The maximum percent inhibition of linoleic acid peroxidation