Confirm manual entries with ENTER. Exit the parameter level at any time by repeatedly pressing the. “Parameter” key. BioPhotometer – Short instructions. Get Eppendorf BioPhotometer Manual. Get all Eppendorf manuals!. Get Eppendorf BioPhotometer plus Instruction Manual. Get all Eppendorf manuals!.
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You can modify most parameters and save them as a modified method see Parameter on page BioPhotometer plus — Operating manual 9 Transport, storage and disposal 9 9. Dependent on the method the results can be calculated via fixed factors, standards, or curve calibration.
The kit contains three filters “sample A1”, “sample A2” and “sample A3” to check the photometric acccuracy and two filters “sample nm” and “sample nm” to check the accuracy of the wavelength.
Similar dimensions as classic cuvettes. BioPhotometer plus — Operating manual 5 Operation 5. To select the results. For specific methods, additional measurements are recorded and displayed as a graph, making impurities easier and quicker to identify.
Eppendorf BioPhotometer : Manual
It biophotomete possible that reduced purity may be attributed to the fact that the sample is solubilized in water instead of in buffer. Eppendorf macro Vis Cuvetteplastic cuvette for measurements in the Vis range, max. Easy and intuitive sample processing. In addition, all important raw data pertaining to the DNA sample are displayed in table format.
Using the cursor keys select the desired method. It is not provided for protein methods. Sample dilutions can also be included in the evaluation.
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Parameter on page Page 23 BioPhotometer plus — Operating manual 5 Operation 5. In biophotometsr to the results the device also displays the absorbance values and some other important details, e.
BioPhotometer plus — Operating manual 4 Installation 4 4. UV-vis is your standard? Your manual failed to upload Don’t see a manual you are looking for?
Eppendorf BioPhotometer User Manual
In general, the purity of a DNA solution is easily defined using purity ratios. Checking condition mahual delivery Set-up and initial operation Instruction of user Self test where applicable IQ Certificate. For details of the measuring procedure via standard one-point calibrationplease refer to “Measuring proteins with reagent”.
Linear and non-linear regression Evaluation procedure of the multi-point calibration. The sample number can be changed as desired e.
Further information about the Eppendorf PhysioCare Concept: Your manual failed to upload Forgot password or username? Page 19 BioPhotometer plus — Operating manual 5 Operation 5.
Page 1 B These packs allow a safe storage of the UVettes in a reclosable box, while offering convenient access to each cuvette for easy filling. Front and rear view Fig. BioPhotometer plus — Operating manual 5 Operation 5 5. The absorbances of the filters are measured against a blank filter “blank A0”. North Description America The result is nanual taking into account the sample dilution entered.
Calculating the molar concentration from the mass concentration and the relative molar mass. You can modify these parameters.
Entries Explanation Calculation Selection Selection of calculation procedures: In practice, nucleic acids are purified with the help of commercially biophotpmeter kits which allow for the separation of most cellular components. For calculating the FOI you can select between 2 units in the method parameters: When the result is calculated and displayed, the dilution factor is included automatically. RNA and valuable samples Deep-lying optical window prevents scratches Tapered cuvette base for optimal filling, frosted grip for labeling Ideal for use in the Eppendorf BioPhotometer and Eppendorf BioSpectrometer, adapters available for use with other common spectrophotometers Routine pack for convenient access to each UVette and safe storage in a reclosable box.
The molar mass biophohometer either entered directly or calculated by the device from the entered figure of the bases or base pairs per nucleic acid molecule.
Page 31 BioPhotometer plus — Operating manual 6 Parameter and functions 6. Partial correction of distortions of absorbance due to turbidity in the measuring solution. Because this is a stray-light measurement the result depends on the geometry of the light path which can differ for the photometers by different manufacturers. Service product portfolio may vary according to country.