BioScope Resolve. The Most Advanced AFM for Life Science Research. Andrea Slade, PhD. Senior Life Sciences Applications Scientist. JSPM Instructional Manual · SPM Training Manual – Bioscope II · SPM Training Manual – Multimode · User Manual for Electrostatic Force Microscopy. Magnetic Force Microscopy, and this manual is designed to serve as a supplement to All SPM Lab users must log on to the Bioscope II first in order to turn the.
|Country:||Trinidad & Tobago|
|Published (Last):||17 October 2009|
|PDF File Size:||10.80 Mb|
|ePub File Size:||13.12 Mb|
|Price:||Free* [*Free Regsitration Required]|
The cantilevers are tip side up as viewed in the wafer holder. Check scan parameters Check that the various scan parameters such as Scan rate, Scan size, Integral gain, etc. Otherwise, it should be left at 0.
Readers may search for these icons to locate information on the imagery of interest. In addition to imaging techniques, the NanoScope software and BioScope system provide the ability to measure fundamental interaction forces between the SPM tip and sample surface Force Cal and Force Step commandsor to use the SPM tip as a microanatomical tool to create nanometer scale patterns in a sample Lithography.
Each of the image Channel control panels Channel 1, 2, and 3 contains a Data type parameter specifying the type of image to be shown on that channel. Oil immersion is usually done with cover glasses.
BioScope™ Instruction Manual |
Slowly adjust to a more positive value until the tip is back on the surface. The computer then tests for true engagement as follows: Der Laser sollte niemals an die Mikroskopelektronik angeschlossen werden, wenn er nicht in der Halterung der ZVerschiebeeinheit installiert ist. Optimization of Scanning Parameters Careful selection of the scan parameters is important to the successful application of contact AFM.
This symbol identifies a laser hazard.
BioScope™ Instruction Manual
Start with bioscopw Integral gain set to 0. Note that the laser sum signal must be greater than 0. Be careful to verify that the bottom bioscppe the sample is NOT being observed. A diagram of the affected pixels appears as shown here: When this process is done, change to the C: Soon after its introduction, a method was sought which would allow non-conducting samples to be imaged.
Introduction Tighten rear adjustment screws until they are brought snug against the microscope frame.
In this instance, a stiff crystal silicon probe is oscillated to its resonant frequency. Connector in back Release Knob Figure 5.
Bioscope Manual for SWI – TINATOONS
The initial Cantilever Tune panels will appear Decreasing the value of this parameter shifts the force curve on the display to the left, while increasing the parameter shifts the curve to the right.
Dimension head in shipping box. Introduction the release screw CCW; D gently lower the head back down in its mount until it comes to rest against the screw. Set the Sweep graph range to the maximum value. Note that there is only one way that the four sockets on the tip holder will align with the pins on the scanner cap: Remove the Pyrex strips by resting the silicon ring on a glass slide or ruler, then applying downward pressure with the tweezers until the strip breaks free from the silicon ring.
Bioscope Manual for SWI
The lower-left screw moves the laser spot rightto-left as shown on the computer screen detector graphic and top-to-bottom short axis on the dark red laser spot screen directly on the SPM head.
The feedback system compares the Manuap amplitude to the setpoint voltage. EFM— Electric force microscope; electric force microscopy. A small Z piezo movement.
Set Z range to a reasonable value for the sample. Fluctuations in current between the STM tip and contact probe were used as the feedback mechanism and to render an electronic image. Probes Probes come in a variety of sizes, shapes and materials and are chosen according to the type of imaging to be done.
The Dimension head is very effective in imaging large samples too large or heavy to scan by movement of the sample. The laser spot will need to be approximately centered prior to entering the Cantilever Tune routine. Cover the petri dish when not in use. Lenses can easily be raked along the underside of the stage plate as they are rotated into position by the turret, or when pressing the sample slide upward into the probe see Figure 3.
In this case, fully tighten both laser aiming screws CWthen unscrew CCW both screws one full turn. Verify that Input attenuation is set at 1x on the Other Controls panel before imaging.