El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Analysis of intra-specific somatic hybrids cutlivo potato Solanum tuberosum using simple sequence repeats. What do you want to do? Gels were analyzed and photographed under UV light. The best study guides. Literature cited Austin S.

For the obtained somatic hybrid plants, the chromosome number varied from Table 2. These bands also appeared in the fusion regenerants Figs. When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking. Avoid resits and achieve higher grades with the best study guides, textbook notes, and class notes written by your fellow students.

After that, colonies were dispersed into plates with solid MS13K regeneration medium Benke, Plant Cell Rep 3: Further work is needed to evaluate their resistance level to Phytophthora infestans Mont. The latter has a natural resistance to late blight, Phytophthora infestans Mont.

When colonies developed into microcalli, the alginate beads were depolimerized.

Somatic hybridization in the genus Solanum: Plant Cell Tissue Organ Cult. Potato, Solanum tuberosum L. Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement. The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed in morphological characters and chromosome numbers among regenerant plants.


Under these conditions, calli hybrids follow the developmental pathway of the morphogenic parents.

Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato Solanum tuberosum L. Thanks to the study guides written by fellow students specifically for your courses, you will never miss a trick when it comes to your exams.

A slot-blot hybridization method for screening somatic hybrids. In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. Due to that, fused and unfused protoplasts were cultured df. Molecular genetic characterization of plant somatic hybrids. Shoot regeneration started twelve weeks after calli were transferred to MS medium.

Isolamento e cultivo de protoplastos de porta-enxertos de citros

Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.

Chromosome observations indicated that all hybrids were aneuploids. Protoplasts were precipitated by centrifugation at rpm during five minutes. Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis. Identification of somatic hybrids of dihaploid S.

Instpp Somatoclonal variation in potato: The same primer amplified four specific bands of, and bp in S. An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al. Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established. Fe other parent, the wild culttivo S.


Fusion-treated protoplasts were cultured in V-KM medium supplemented with bovine serum albumin. It has been demonstrated that protoplast-derived plants show a higher degree of chromosomal number variation than those derived from tissue or organ culture.

The mixture was incubated for thirty minutes and then protoplasts were collected by centrifugation at rpm during five minutes. This technique is a tool for somatic hybrid characterization Pprotoplastos et al. Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum species.

Tema 9. Cultivo de protoplastos e hibridación somática

Your fellow students know exactly where the pitfalls lie and what the key elements will be to your success in that module. With the study guides and notes written by fellow students, you are guaranteed to be properly prepared for your exams. Protoplaztos this analysis, DNA extraction was performed according to Doyle and Doyle with some modifications.

Interspecific somatic hybridization between Solanum tuberosum L. Production of somatic hybrids between frost – tolerant Dee commersonii and Solanum tuberosum:

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