Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.

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Fourth, cryovials were thawed as described above; root tips were washed with original culture medium and plated on the solid medium without filter paper. K-MO-C had an original idea of the stability and cryopreservation work, designed the work and wrote the manuscript together with SH.

Plant Cell Tissue Organ Cult. Although the growth rate and hyoscyamine production had stayed stable, the scopolamine production was more than double compared to original data. Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus cutlure L. Extraction of tropane alkaloids hairy roots contained the rol genes rol B, rol Crevealed by hyoscyamine and scopolamine was based essentially on the PCR analysis Fig.

In this study, the alkaloid production of the one particular cultute root clone was measured in, and Hyosycamus Agrobacterium tumefaciens strain C58C1 harbor- ing both pBMI and Agrobacterium rhizogenes Ri plasmid pRiA4, containing a single pmt gene, were used for plant transformation 16, The hy- few genera of the plant yhoscyamus Solanaceae, including Hyoscyamus, droxylase gene from Hyoscyamus niger has been introduced into Duboisia, Atropa, and Scopolia, are able to produce biologically Atropa belladonna, a typical hyoscyamine-rich tropane alkaloid- active nicotine and tropane alkaloids simultaneously 1—3.

Epigenetic aspects of somaclonal variation in plants.

Enter the email address you signed up with and we’ll email you a reset link. Plant alka- loids constitute the largest groups of natural products, providing ratio cuture scopolamine to hyoscyamine in scopolamine-producing cultured roots 9. Somaclonal variation in transformed roots and protoplast-derived hairy root clones of Hyoscyamus muticus.


T-DNA, portion of the Ti tumor-inducing plasmid that is transferred to plant cells.

The pXI was isolated from E. Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6beta-hydroxylase. Accumulation of tropane alkaloids and growth of H.

This work also sheds light on how to plays a more important role in stimulating scopolamine accu- effectively increase the end products of secondary metabolic path- mulation whereas the proportional functioning of the upstream ways by appropriate genetic engineering strategies.

However, for organized tissues such as hairy roots shown in muticud study, long-term subculturing was successfully applied with genetically engineered roots without significant loss of enzymatic or metabolic activity. Decades of hoscyamus work has turned this hairy root disease into a hjoscyamus biotechnological application Georgiev et al.

Finally they can be detached and grown as independent hairy root clones. Hairy root is caused by a plant disease through the infection of Agrobacterium rhizogenes carrying Ri root inducing plasmid. Root tips were thawed in a following way unless otherwise described. Plants remain a major source of new drugs, leads and fine chemicals. Putrescine wild-type hairy roots. After the establishment of the hairy root line 16 years ago Jouhikainen et al.

R Foundation for Statistical Computing. Expression of the rolC gene and nicotine production in transgenic roots and their regenerated plants. Hairy root lines generated from transforma- type, implying the occurrence of cosuppression, which subse- tions with strains A4 and LBA containing no pmt or h6h are quently resulted in the low scopolamine accumulation in this line denoted culturr A4 and LBA, respectively.

An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

No use, distribution or reproduction is permitted which does not comply with these terms. Thawing was performed using four different procedures, displayed in Figure 1. After 28 days of hyoscyams, the roots were filtered and generated 4-week-old root lines of culture by using TRIzol washed with 10 ml of sterile distilled water and lyophilized Several hyoscyamine-rich but scopolamine-poor plants exploration of metabolic engineering as a potential effective that had been considered unattractive for commercial exploita- approach to increase the yield of specific metabolites by enhanc- tion may now become promising candidates for large-scale ing rate-limiting steps or by blocking competitive pathways.


Scopolamine Hydrobromide Search for additional papers on this topic.

An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

About derived from transformation T lines by using the same PCR mg of fresh roots 3 cm in length were inoculated into ml conditions for the detection of the h6h gene. Enhancement of scopolamine production in Hyoscyamus muticus L. Hyoscyajus freezing method described by Schmale et al.

Comparison of plant-based expression platforms for the heterologous production of geraniol. In certain cases the production of secondary metabolites in plants is limited rooh specialized tissues or organs and the capability is lost in undifferentiated cells. Small-scale jar fer- enzyme H6H plays a haigy important role in stimulating scopol- menters for the hairy roots of several Solanaceous species have amine accumulation in H.

Although the exact mechanism of rol gene —mediated induction of secondary metabolite biosynthesis is not known, it is thought that induction involves the function of signal transduction pathways, including interaction with proteins and tyrosine phosphatase Moriuchi et al.

Log In Sign Up. Cell cultures offer advantages to produce cell biomass or metabolites in a controlled environment.

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