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Structure of a chromosomal gene for human interferon beta. Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA irv some cytokines. Regulation of gene expression by interferons: Open in a separate window.

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Please review our privacy policy. Associated Data Supplementary Materials. Reversible silencing of enhancers by sequences derived from the human IFN-alpha promoter.

High efficiency DNA-mediated transformation of primate cells. Short and long range activation by the SV40 enhancer.

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex es with the regulatory elements. Support Center Support Center.


A nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus. Heat shock factor is regulated differently iirf yeast and HeLa cells. Delimitation and properties of DNA sequences required for the regulated expression of human interferon-beta gene. Tissue-specific expression of the human growth hormone gene is conferred in part by the binding of a specific trans-acting factor.

In this study, we describe a nuclear factor, termed interferon regulatory factor-1 IRF-1that is 410 in the transcription of IFN-beta and possibly other genes. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. A rapid and sensitive assay system for bacterial gpt activity in transfected mammalian cells. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.

Changes of chromatin conformation around mouse interferon-beta gene associated with induction of interferon synthesis. National Center for Biotechnology InformationU. Abstract Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines.

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Biochemistry of interferons and their actions. Author information Copyright and License information Disclaimer. Establishment of a permanent cell line HEL-R66 from human embryonic lung cells with high susceptibility to viruses. IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells.


The human beta-interferon gene enhancer is under negative control. Human embryonic lung cells HEL-R66 are of monkey origin.

Affinity purification of sequence-specific DNA binding proteins. Sequencing end-labeled DNA with base-specific chemical cleavages. Interaction of positive and negative regulatory proteins in the galactose regulon of yeast. Human beta-interferon gene expression is regulated by an inducible 461 element. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.

BK viral enhancer element and a human cellular homolog. Images in this article Image on p.

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Intracytoplasmic phosphorylation sites of Tac antigen p55 are not essential for the conformation, function, and regulation of the human interleukin 2 receptor.

The 5′-flanking sequence of human interferon-beta 1 gene is responsible for if induction of transcription. Regulation of cytokine gene expression. Controlled transcription of a human alpha-interferon gene introduced into mouse L cells.

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