Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples. Keratinase is an inducible enzyme that is synthesized only when an inducer ( keratin) appears in the environment. Keratinase can be produced by many kinds of. The three Bacillus spp. produced extracellular keratinases and of feather or feather meal on the production of keratinolytic enzymes by three.
|Published (Last):||26 July 2007|
|PDF File Size:||18.45 Mb|
|ePub File Size:||16.45 Mb|
|Price:||Free* [*Free Regsitration Required]|
Microbial Keratinase Production and Application to Improve the Properties of Wool Fabrics
Keratinase production was more than 5. Higher amount of keratinase kerainase was found to produce by Arthrobacter sp. A modified method for the detection of microbial proteases on agar plates using tannic acid.
The dyes used were kindly supplied by DyStar Company and the commercial protease enzyme was supplied by Novozyme. Properties The molecular weight of keratinase that has been isolated and purified is quite different, ranging from dozens of kDa to several hundred kDa.
Feather degradation with a thermotolerant Streptomyces graminofaciens strain, World J.
These dehairing chemicals contain a high amount of pollutants such as soda-lime, salts, chromate, sulphide, solvent flashy, emulsified fatty matter, waste lime liquor. The optimum pH for keratinase production by Arthrobacter sp. In biochemical characters, both organisms were catalase positive and produced H 2 S.
Keratin is also categorized as hard and soft keratin comprising the amount of sulphur in their amino acids content. The isolates showing moderate to high keratinolytic activity were tested for the production of keratinase enzyme in basal media. Keratinases are projected to generate a potential worldwide market similar to other proteases. Two isolates BF11 and BF21 exhibiting maximum keratinase were selected and the keratinase production was compared in three liquid media.
Results and Discussion A total of 80 samples prroduction analysed for potential keratinase producing organisms.
The presence of the products of sulphitolysis such as peptides, cysteine, cysteine, and sulphate in the culture filtrate of MBF isolates confirmed the disulphide breakdown. The three microorganisms were able to produce keratinases and peptidases after 7 days of fermentation.
Three Bacillus species B. The pH and volume of the media were 7.
Pdoduction results identified starch, galactose, and glycerol as good carbon sources in the order mentioned. The mostly used enzymes, keratinase and protease, preferentially pgoduction the disulphide cross linkages between fibers imparting hydrophilicity, dyeing and shrink resistance to wool fabrics.
View at Google Scholar H. The present study reports the use of these approaches to characterize and improve keratinase production by two Bacillus sp. The time required for the water drop to disappear was measured and recorded as wetting time. Authors are grateful to Microbial Biotechnology Division, National Institute of Biotechnology for providing research facilities.
Keratinase – Creative Enzymes
In previous researches, keratinase production was performed by generally feather-degrading microorganisms therefore; there are very limited studies about wool-degrading microorganisms [15, 21]. Biochemical features of microbial keratinases and their production and applications, Appl. Zymograms The concentrated culture supernatant was mixed with the peptidase sample buffer [Tris-HCl 0. Feather meal was the best substrate for keratinase production with B.
Alteration of the keratinase activity according to the time. The tendency of the medium to turn alkaline has been attributed to deamination reactions leading to production of ammonia from protein, peptides, and amino acids during keratin degradation. The soil samples ekratinase collected from Hazaribagh tannery industry, Dhaka and local poultry farms of Savar, Dhaka. In fungi, keratinase producers are generally relating to dermatophytes comprising MicrosporumTrichophytonChrysosporiumGeomyces .
The bacterial isolates were further sub-cultured to obtain pure culture. Application of rDNA technology to increase the yield of keratinase had limited success. This may be due to the fact that the cysteine -SH produced by the reduction of the cystine disulfide bond was quickly converted to other product.
Biochemical and molecular characterization of a new local keratinase producing Pseudomonas sp. Keratin fibers from chicken feathers: Screening and production of extra cellular feather degrading enzyme from Bacterial isolates.
In each case, lower amount of keratinase production was achieved from Peptone containing media.
Results and Discussion 3. All experiments were carried out five times. Various authors have reported that, among the keratinolytic microorganisms, some species of Bacillus [ 10 — 12 ], actinomycetes [ 91314 ], and fungi [ 15 — 17 ] are able to produce these keratinases and peptidases.